Phylogene Specialized Analyses Laboratory Mass Spectrometry

Technologies

Mass spectrometry :

Mass spectrometry is a physics analytical technique that detects and identifies molecules by measuring their mass, and characterizes their chemical structure. Its principle is in the separation of charged molecules in an electric field in function of the ratio mass/charge (m/z)

A mass spectrometer is made up of one ionisation source which ionizes and sprays the components, one m/z analyser  (MS analysis) or one analysers combination  (MS/MS analysis) , one ions detector and one signal treatment system. These instruments are characterized by their ionisation mode, their mass accuracy and their resolution.  Some ionization sources ( ESI Electrospray Ionization) can be directly coupled to a separation method like the liquid chromatography in the order to increase the analysis resolution.

Liquid chromatography :

High performance liquid chromatography is an analytical separation technique in function of physico-chemical mixed molecules properties, like hydrophobicity, net charge or steric size. Its principle is based on the affinity difference of a component between a stationary phase and a mobile phase which affinity increases with the processing time. The instrument used is a HPLC chain which includes a pump that pushes the mobile phase in the column, an injector that injects the sample in the column, a column which contains the stationary phase and a detector which in our case is a mass spectrometer.

Installations

Positive pressure laboratory to protect samples during extraction.

Type II Safety cabinet to work microbial strains.

Equipment

High resolution mass spectrometers.

High-performance liquid chromatography chain at micro and nano flow.

Multi dimensional liquid chromatography

Micro fluidic separation system.

Bioinformatics

Mass spectrometry

Mascot, Skyline and Paragon search engines for protein identification against databases.

Protein Pilot 4.0 for iTRAQ differential analysis

Marker View for label free differential analysis

MRM Pilot and Multi Quant for targeted experiences optimization and treatment

Bioinformatics and data processing

CORAVALID modules