Phylogene Specialized Analyses Laboratory Mass Spectrometry

Services

Example of service

- Identification and relative abundance of components (label-free nanoLC-MS/MS) : ingredient characterization
Non-targeted technique for identification and quantitation of 50 most represented proteins in a proteome

- Identification and relative label-free quantitation of samples (label-free nanoLC-MS/MS): biomarkers search, biodiscovery, effect discovery.
Non-targeted and exhaustive technique at the level of 2/3 to 3/4 of whole cell proteome

- Bioinformatics/biostatistics dedicated analysis (CORAVALIDTM)
Technique which favorably allows to exploit all information for a good understanding of phenomenons.
http://ms.phylogene.com/Local/msphylogene/files/33/NEWS.6.Neurological.diseases.proteomics.pdf
http://ms.phylogene.com/Local/msphylogene/files/82/Effect.study.example.112016.pdf
http://ms.phylogene.com/Local/msphylogene/files/91/Voegeli_et_al-2017-International_Journal_of_Cosmetic_Science.pdf
http://ms.phylogene.com/Local/msphylogene/files/83/NEWS.29B.FFPE.proteomics.to.enhance.diagnostic.biomarker.and.therapeutic.target.discovery..pdf
http://ms.phylogene.com/Local/msphylogene/files/53/NEWS.22.Serum.proteomics.biomarker.in.eye-s.diseases..pdf

- Bioinformatics/biostatistics dedicated analysis of the microbiome (MicroXploreTM)
Technique which, in addition to CORAVALIDTM for the host, allows to process the microbial proteome data at the functional level, in studies of effects on host and microbiota.
http://ms.phylogene.com/Local/msphylogene/files/94/NEWS.39.Functional.studies.for.human.and.microbiome.interactions.pdf
http://ms.phylogene.com/Local/msphylogene/files/93/NEWS.41.Proteomic.and.metaproteomic.approaches.to.understand.host-microbe.interactions.pdf
http://ms.phylogene.com/Local/msphylogene/files/88/Microbiome.Active.ingredient.study.012016..pdf


- Perfecting and using of multiplex targeted methods (Multiple Reaction Monitoring)
Technique which is independent from available or to be developed antibodies, and allows to assay several 10th of proteins simultaneously
http://ms.phylogene.com/Local/msphylogene/files/49/NEWS.17.Multiplex.SRM.method.or.ELISA.pdf

-Phosphoproteomics
Serine/threonine mono- and polyphosphorylated phosphoproteins
PTMs modifications after phosphoproteins enrichment

http://ms.phylogene.com/Local/msphylogene/files/52/NEWS.19.Phosphoproteomics.to.characterize.cells.or.biopsies.in.cancer.pdf

- PTMs
Isoforms Identification (acetylation, oxidation,methylation, ubiquitination…) and comparison by relative relative quantification with nanoLC-MS/MS

-Metabolomics
Mainly through proteomics, label-free relative quantification of samples or targeted quantification

-Kinome profiling
In untargeted mode, which kinase is under or over expressed in the tissue
-Kinome inhibition profiling
Comparison of conditions with and without inhibitor in an untargeted mode

-Drug inhibition measurement
IC50 of all identifiable kinases in an untargeted mode
http://ms.phylogene.com/Local/msphylogene/files/38/NEWS.12.Cancer-.kinome.and.kinase.inhibitors.studies.pdf

Prices

References

Service

Unitary price ¤

Details

00912-01

Proteins identification : shotgun proteomics

(1-5 samples)

220(1)

Digestion, dessalting by SPE, LC-MS/MS analysis, databases query

00912-02

Proteins identification

 (6  samples or more)

190(1)

Digestion, dessalting by SPE, LC-MS/MS analysis, databases query

00912-03

Whole protein exact molecular weight determination (2)

280

On line dessalting, LC-MS/MS analysis, spectra deconvolution, exact mass determination, micro heterogeneity analysis

00912-04

MRM method quantification

Contact us

Digestion, dessalting by SPE, LC-MS/MS analysis, quantification by isotopic labelled standard range

00912-05

MRM method development

Contact us

Target determination by in vitro and in silico approach , transition determination, physico-chemical parameters optimization, detection and quantification limits determination

00912-06

Differential analysis , relative quantification by label-free method

Contact us

Digestion, dessalting by SPE, LC-MS/MS analysis, data statistical treatment

00912-07

Differential analysis , relative quantification by iTRAQTM method

Contact us

Digestion, iTRAQTM labelling ,dessalting by SPE, LC-MS/MS analysis,relative quantification

00912-08

protein identification

by 2D-LC-MS/MS on line

Contact us

Digestion, dessalting by SPE, LC-MS/MS analysis, databases query

00912-09

Quantification by SWATH-MS method

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Digestion, dessalting by SPE, LC-MS/MS analysis, components quantification

 

  1.  Applicable to protein extracts, contact us for any other sample type
  2.  Applicable only to purified proteins

 

Any service will be formalized by a quotation that will precise :

  • Type of analysis
  • Results transmission practical details ,
  • Analysis allotted time,
  • Price,
  • Sample size to be sent,
  • Sampling parctical details,
  • Information for samples delivery,

At samples arrival, you will be informed of anything not conform to the order

 

A contract may be drawn up according to your needs. (lien vers formulaire de contact)

Quality

Phylogene is an ISO 17025 accreditated laboratory by COFRAC (n° 1-2128).

Phylogene is member of V03E et V03B AFNOR standardization commissions as well as CEN WG12 working group on the allergens (lien vers Qualité site Phylo)

Competitivity

Our continuous care is to offer you the shortest delays and the best prices. The prices are adapted to the samples number.

 (lien vers formulaire de contact)